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KMID : 1025520070490030295
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Journal of Animal Science and Technology
2007 Volume.49 No. 3 p.295 ~ p.302
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Analysis of Single Nucleotide Polymorphisms of Leptin Gene in Hanwoo (Korean Cattle)
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Lee Jeung-Min
Song G. C.
Lee J.-Y.
Kim Young-Boong
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Abstract
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Leptin, the product of the obese(ob) gene, is an adipocyte-derived hormone for the regulation of whole- body energy storage and energy usage. It has been reported that the homozygous mutations in the gene for leptin(LEP) induce obesity and reduce energy expenditure. In cattle, LEP has significant roles directly or indirectly related with phenotypes such as body weight and fat deposits, therefore SNPs of LEP have been considered important genetic marker to estimate carcass fat content in cattle. In this study, SNPs were screened in LEP(2,222 bp) between intron 1 to 3’-UTR from 24 independent Hanwoo(Korean cattle) by PCR and DNA sequencing. Total 25 SNPs were found and two nonsynonymous SNPs including T1163A(V19E) and G3256A(G132D) were newly detected only from Hanwoo. Among 20 SNPs previously reported in cattle, 16 SNPs were found in Hanwoo; however, the frequencies of some SNPs were significantly different between Hanwoo and western cattle breeds. The other 4 SNPs were not detected from Hanwoo. These Hanwoo specific SNP patterns in LEP will be used in development of molecular marker and application to genetic improvement of Hanwoo.
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KEYWORD
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Leptin, Single nucleotide polymorphisms, SNPs, Hanwoo (Korean cattle)
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